Facilities

Preservation Protocol Development:

Cell culture:

  • Biological safety cabinets (BSL 2)
  • oC fridges
  • CO2 incubators
  • Centrifuge (micro, desktop and floor models, volumes from 100 μl to 1 L)
  • Milli-Q water purification system
  • Autoclave

Preservation:

  • Planar 16 controlled rate freezers
  • MVE liquid nitrogen storage units with monitoring systems
  • -20 oC mechanical freezer
  • -80 oC freezer
  • Dry shippers
  • Brymill liquid nitrogen sprayers
  • Mr. Frosty
  • Corning CoolSink and CoolRack

Liquid Handling:

  • Precision PRC384 liquid handler
  • Eppendorf 5804R refrigerated centrifuge
  • Thermo-Fisher Multidrop Combi dispenser
  • Velocity 11 Plate Loc plate sealer
  • Nanoliter PocketTip compatible multi-channel pipettes

 

Imaging and Analytical:

Imaging:

  • Zeiss upright microscope
    • Fluorescent and phase contrast imaging capabilities
    • Low light Chip camera
    • SPOT image acquisition software
  • Nikon microscope with phase contrast
  • Leica microscope with polarized light capabilities
  • Stereo/dissection microscope
  • AmScope 18 megapixel CMOS digital camera
  • NE18 reticles
  • Triple and Quadruple Peltier Thermal stages with controller
  • Vibration isolation table

Analytical:

  • BioTek Synergy HTX Multi-Mode Plate Reader
  • LINKAM FTIR600 temperature controlled imaging stage
  • BioRad spectrophotometer
  • Osmette osmeter (freezing point based)
  • NMR tubes and coaxial inserts
  • Analytical balance (0.001 gram resolution)
  • pH meter
  • Conductivity meter
  • Controlled heating-stir plates

 

Automation:

  • Directional solidification jigs (accommodates approx. 1.5 inch wide samples)
  • DATAQ thermocouple data logging (DI-245, DI-2008)
  • Adjustable benchtop power supplies
  • Electronics toolkit

 

Incubator (culture) - Wikipedia

 

 

Controlled Rate Freezer | Programmable Freezer | Kryo 560-16 | Planer PLC

 

 

 

 

Preserving cells: Minnesota startup 'BlueCube Bio' develops new approach |  kare11.com

 

 

UMN Offers 10% Off to Members of ARMI | BioFabUSA on "Preservation of  Cellular Therapies Course" — Advanced Regenerative Manufacturing Institute

 

Linkam FTIR600 Heating and Freezing Stage

Multi-detector microplate reader - Synergy™ HTX - BioTek Instruments