Preservation Protocol Development:

Cell culture:

• Biological safety cabinets (BSL 2)
• 4 ^oC fridges
• CO₂ incubators
• Centrifuge (micro, desktop and floor models, volumes from 100 μl to 1 L)
• Milli-Q water purification system
• Autoclave

Preservation:

• Planar 16 controlled rate freezers
• MVE liquid nitrogen storage units with monitoring systems
• -20 ^oC mechanical freezer
• -80 ^oC freezer
• Dry shippers
• Brymill liquid nitrogen sprayers
• Mr. Frosty
• Corning CoolSink and CoolRack

Liquid Handling:

• Precision PRC384 liquid handler
• Eppendorf 5804R refrigerated centrifuge
• Thermo-Fisher Multidrop Combi dispenser
• Velocity 11 Plate Loc plate sealer
• Nanoliter PocketTip compatible multi-channel pipettes

Imaging and Analytical:

Imaging:

• Zeiss upright microscope
  • Fluorescent and phase contrast imaging capabilities
  • Low light Chip camera
  • SPOT image acquisition software
• Nikon microscope with phase contrast
• Leica microscope with polarized light capabilities
• Stereo/dissection microscope
• AmScope 18 megapixel CMOS digital camera
• NE18 reticles
• Triple and Quadruple Peltier Thermal stages with controller
• Vibration isolation table

Analytical:

• Countess 3 FL Automated Cell Counter
• BioTek Synergy HTX Multi-Mode Plate Reader
• LINKAM FTIR600 temperature controlled imaging stage
• BioRad spectrophotometer
• Osmette osmeter (freezing point based)
• NMR tubes and coaxial inserts
• Analytical balance (0.001 gram resolution)
• pH meter
• Conductivity meter
• Controlled heating-stir plates

Automation:

• Directional solidification jigs (accommodates approx. 1.5 inch wide samples)
• DATAQ thermocouple data logging (DI-245, DI-2008)
• Adjustable benchtop power supplies
• Electronics toolkit